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J. gen. Virol. 2: 123-134, 1969. Stoltz, D. B. J. Ultrastruct. Res. 43: 58-74,1973. , Dove, M. , Lee, P. , and Seligy, V. L. Biochem. Cell BioI. 64: 495-503, 1986. Bellet, A J. D. and Inman, R. B. J. Mol. BioI. 25: 425-432, 1967. B. A cytological and biochemical study of Estigmene acrea continuous cell cultures infected with TipuZa iridescent virus. Sc. Thesis, Carleton University, Ottawa, Canada, 1978. Kelly, D. C. Curr. Top. Microbiol. : 23-35, 1985. Hall, D. W. Viral insecticides for biological control (Eds.

Similar models have been suggested for adenovirus and herpes virus assembly (63,64). It is evident that a large amount of information has accumulated on the gross structural properties of TIY in the last three decades. These studies have increased our understanding of viral architecture and have helped in the creation of models to explain the icosahedral design (6). However, aside from these pioneering studies, little or no molecular information has been produced to explain the mode of replication, pathogenesis, and the regulation of gene expression of this and other iridescent insect viruses.

Nielsen and Brown (140) suggested that the different shades of blue, yellow, red, and gray that characterize different proteins stained wi,th silver, can be predicted if the amino acid sequence is known. Further, they suggested that the color of protein/silver complexes is related to the binding of silver to the side chains of charged amino acids. In contrast, Chuba and Palchaudhuri (141) showed a strong correlation existed between increased staining with silver and the cysteine content of the protein.

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